里岔黑猪GDF11基因cDNA的克隆及组织表达谱分析

摘要

生长分化因子11(Growth and differentiation factor-11,简称GDF11)是BMP/TGF-β超家族一员,编码一种在早期胚胎发育过程中起重要作用的骨形态发生蛋白,在确立骨骼模式中起重要作用.本研究旨在克隆里岔黑猪GDF11基因部分cDNA,并检测其在不同组织中的表达差异.结果表明,所克隆的猪部分GDF11基因序列为1 161 bp( GenBank:HQ657211),编码332个氨基酸,与人、小鼠、大鼠、牛、马、兔、斑马鱼GDF11氨基酸序列同源性分别为99.10％,99.10％,99.10％,99.40％,99.10％,98.80％,78.82％.荧光定量PCR分析结果显示,GDF11基因mRNA在14种组织中均有表达,总体趋势为:脊椎＞背膘＞膀胱＞肺＞脾＞胆囊＞肾＞大肠＞心＞骨骼肌＞肝＞小肠＞眼肌＞胃.这为进一步研究里岔黑猪GDF11基因的结构和功能及其脊椎数发生的机理奠定了基础.%GDF11 , also known as bone morphogenetic protein 1 1 ( BMP11 ) , is a member of the transforming growth factor-β superfamily and BMPs subfamily. It is a secreted protein which is considered to be a key regulator of axial skeleton patterning during early embryogenesis. In this study, the partial cDNA of porcine GDF11 gene was cloned by RT-PCR and the expression patterns of GDF11 gene were detected. Sequence analysis indicates that the sequences of porcine GDF11 gene were 1161 bp in length( GenBank : HQ657211. 2) . The cloned cDNA encoded a protein of 332 amino acid residues, which shared 99. 10% ,99. 10% ,99. 10% ,99.40% ,99. 10% ,98. 80% and 78.82% identity with those of human , mouse, rat, cattle , horse , rabbit and zebra fish , respectively. Expression pat-terns of GDF11 were detected in 14 different tissues by real time quantitative RT-PCR, and patterns of GDF11 gene,as a whole,tended to be spinal cord ＞ backfat ＞ urinary bladder ＞ lung ＞ spleen ＞ gallbladder ＞ kidney ＞ large intestine ＞ heart ＞ skeletal muscle ＞ liver ＞ small intestine ＞ longissimus Dorsi muscle ＞ stomach. These results provide important information for further elucidating the molecular biology functions and mechanism of GDF11 gene.