Method for preparing a DNA containing a complete copy of the Genome RNA of the coronavirus in the form of a cDNA which is capable of generating an infectious viral RNA, infectious clone,Bacteria and viral vector comprising this infectious Clone Vaccine comprising, in addition to the viral vector.

摘要

Methods for preparing DNA involves the cloning of DNA that includes a copy of the full length genomic RNA (ARng) of an RNA virus; or (b) a good DNA comprising oneSeveral Fragments of an ARng or a virus RNAWhere such Fragments encodes an RNA dependent rna polymerase and at least a Structural or non Structural Protein; (c) a DNA that is at least 60% Homology with the SECInflows of (a) or (b); in a bacterial artificial chromosome (BAC).In addition, we provide DNA comprising sequences derived from genomic RNA of the coronavirus, (ARng)Where such sequences have Homology of at least 60% with the natural sequence of virus encoded RNA dependent rna polymerase a and at least one Protein StructureUral or not Structural, where a fragment of this DNA has the capacity of transcribed RNA, where DNA can be assembled in a virion.In addition, it describes the use of these Nucleic Acids in the preparation of viral RNA or virions as well as Pharmaceuticals. These preparations include viral DNA, RNA or virions.