阿巴嘎黑马染色体核型及G带分析

摘要

In this paper ,chromosomal karyotype of Abaga horse was studied by using the fibroblast cells culture method and conventional chromosome analysis techniques .The karyotyping of Abaga horse has been analyzed by standard and G-banding technique .The results showed that the diploid chromosome number of Abaga horse was 2n=64.The chromosome karyotypes of male and female horse were 64,XY and 64,XX.The chromosome 1-13 is metacentric-submetacentric autosome;chromosome 14 -31 is acrocentric autosome .Chromosome X is the second longest submetacentric chromosome and Y is the smallest acrocentric chromosome .Moreover , each chromosome of Abaga horse has some unique patterns of its bands ,and there are some differences with the reported Araba horse . This is the first report of G-banding karyotype of Abaga horse in China .The karyotype generated in this study will provide the fundamental information for equine cytogenetics and diagnosis of genetic disease in Arab horses .%采用耳组织成纤维细胞培养方法及常规染色体标本制备技术，对阿巴嘎黑马的染色体核型及G带进行了分析。结果显示，阿巴嘎黑马染色体数目为2n ＝64，雄性核型为64，XY；雌性核型为64，XX。其中1～13号染色体为中或亚中着丝粒染色体，14～31号染色体为端着丝粒染色体，X染色体则为2对大亚中着丝粒染色体，Y染色体为最小的端着丝粒染色体。另外，阿巴嘎黑马染色体G带明暗相间，且每对染色体都有其独特的带纹特征，其中部分染色体的G带与已报道的阿拉伯马的G带特征有明显差异。本研究首次在国内报道了阿巴嘎黑马染色体核型及G带特征，这将为阿巴嘎黑马的细胞遗传学研究和疾病诊断提供资料。