CALCA 基因遗传变异及其与肉质性状关联分析

摘要

The aim was to analyze association of CALCA gene polymorphisms with some economic traits in Zaosheng cattle and Zaosheng crossbred , and to evaluate it as a candidate molecular marker associated with beef quality.PCR-SSCP method was used to detect the genetic variation in CALCA gene in 362 cattle from 5 cattle groups ,including Zaosheng in Qingyang Prefecture , Zaosheng cattle in Pingliang Prefecture , Nanza ( South Devon cattle ×Qingyang groups),Qinza (Qinchuan cattle ×Pingliang groups),Xiza (Simmental ×Pingliang groups).The association between SNP locus and some carcass traits of Pingliang groups were analyzed .Two genotypes AA and AB were detected at exon 4 in CALCA gene .Analysis of association of polymorphism with carcass traits at mutation locus showed that there were significant effects on WBSF ,Ribeye area ,Hot carcass weight and Cooking loss ( P<0 .05 ) and there was no effect on other traits(P>0.05).In conclusion,SNP of CALCA gene could be a genetic mark for carcass trait.%旨在分析早胜牛及其杂交牛CALCA基因第4外显子的多态性及其与肉质性状的相关性，发现与肉质性状相关的分子标记，为加快肉牛选育进程提供参考。利用PCR-SSCP 技术对早胜牛、南杂牛、秦杂牛和西杂牛共362个个体的CALCA基因第4外显子进行了遗传变异检测，并运用SPSS 17．0软件对早胜牛（平凉类群）遗传变异与经济性状关联性进行了分析。结果表明，CALCA基因第4外显子存在2个突变位点T3237 A和G3289 A，并检测到AA和AB 2种基因型。相关性分析表明，CALCA基因突变位点与剪切力、眼肌面积、热胴体重和熟肉率显著相关（P＜0．05），AA基因型个体剪切力、热胴体重和熟肉率显著高于AB基因型个体（ P＜0．05），而AB基因型个体眼肌面积显著高于AA基因型个体（P＜0．05），其他性状在各基因型间差异不显著。因此，初步推测CALCA 基因的突变位点可以作为评定早胜牛肉质性状的遗传标记。