为揭示复等位基因遗传的大白菜雄性不育分子机制,通过基于质谱的蛋白质组学同位素相对标记与绝对定量技术技术( iTRAQ),开展了大白菜核基因雄性不育蛋白质组学研究,以找到不育与可育材料中差异表达蛋白,从蛋白水平来进一步揭示大白菜雄性不育的分子遗传机制。通过研究,共发现了358个差异蛋白,其中可育中上调表达的蛋白有226个,下调表达的蛋白有132个,GO 分析结果表明,鉴定的蛋白质组数据具有较好的生物学功能覆盖范围。通过双向电泳验证,差异蛋白点差异特性与 iTRAQ结果类似,表明 iTRAQ用于差异蛋白分析结果可靠。%To better understand the molecular mechanisms of multiple-allele-inherited male sterility in Chinese cabbage( Brassica rapa L. ssp pekinensis),the iTRAQ quantitation technique based on mass spectrometry were used to study the differencial expression between proteins fertile- and sterile-buds. This research would provide the basis for analyzing the molecular mechanism of multiple-allele-inherited male sterility in Chinese cabbage. In this re-search,a total of 358 differencial expressed proteins(226 up-expressed and 132 down-expressed proteins in fertile buds)were found and Gene Ontology( GO)analysis showed that the proteins identified data has better coverage of biological functions. It is verified by two-dimensional electrophoresis(2-DE). This results is consistent with iTRAQ technique,which indicated that using iTRAQ to analyze protein profile is reliable.
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