The laccase producing medium of Trametes versicolor SYBC L3 by using solid-state fermentation was optimized. The results showed that the optimal soybean meal and sawdust mixed ratio of 1 :1. The optimal carbon source is Maltose 1. 0 % (w/w). The optimal nitrogen is Sodium nitrate 2.5% (w/w). The concentration of copper sulfate CuSO4 is 0. 02%(w/w). The optimized solid-state fermentation condition was 40 g solid-state medium in 250 mL Erlenmeyer flasks. The optimal medial moisture content is 70%(w/w). The optimized pH value of the buffer for crude enzyme extract was 6. 5. The optimized time for laccase extract was 4 h. After be optimized,the laccase of Trametes versicolor SYBC L3 by using solid-state fermentation%对菌株Trametes versicolor SYBC L3产漆酶固态发酵培养基进行优化,确定该菌适宜的发酵条件为豆粕和木屑比例为1∶1,最适碳源是麦芽糖1.0 % (w/w),最佳氮源是硝酸钠2.5% (w/w),硫酸铜浓度为0.02% (w/w).在250 mL三角瓶中装样40 g,含水量为70%(w/w),提取最适pH为6.5,酶曲最适浸泡时间为4 h时,优化后菌株Trametes versicolor SYBC L3固态发酵所产漆酶酶活可达91.057 U/g 干曲.
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