CELL OF MYCELIAL FUNGUS PENICILLIUM THE CANESCENS- PRODUCER OF XYLANASE AND LACCASE, METHOD OF OBTAINING COMBINED FERMENTATION PREPARATION OF XYLANASE AND LACCASE

摘要

1. Cage mycelial fungus Penicillium canescens, transformed with a plasmid containing the DNA fragment encoding the xylanase, and a plasmid containing the DNA fragment encoding the laccase, - producing xylanase and lakkazy.2. The cell of claim 1, characterized in that the said plasmids are plasmids and pBGlac pBGxylD sootvetstvenno.3. The cell of claim 1, which is a cell strain of Penicillium canescens PCA-10-4 / I-7/12 niaD, and transformed with plasmids pBGlac pBGxylD, - C1 ligninolytic laccase producer fungus Trametes hirsuta and xylanase XylD filamentous fungus Penicillium canescens.4. A method of producing a xylanase enzyme preparation and laccase, comprising the steps of growing the cells mycelial fungus Penicillium canescens according to claim 1 in a nutrient medium and isolating the xylanase enzyme preparation and zhidkosti.5 laccase from the culture. A method according to claim 4, characterized in that as a cell strain of filamentous fungus Penicillium canescens using cells of 2.6. A method according to claim 4, characterized in that the cultivation of cells of the strain of claim 1 carried out in the presence metalloproteinaz.7 inhibitors. A method according to claim 6, characterized in that the metalloproteinase inhibitor as used EDTA.8. A method according to claim 4, characterized in that the cultivation of cells of the strain of claim 1 carried out in a medium with a pH ranging 4,0-6,0.9. A method according to claim 4, characterized in that the selection enzyme preparations xylanase and laccase from the culture medium is carried out by concentrating using ultrafiltration membrane with a cutoff threshold of 5 KDa followed by vacuum evaporation of the filtrate of culture liquid at a temperature of 40-70 ° C.