文摘
英文文摘
论文说明:LIST OF FIGURES、LIST OF TABLES、LIST OF ABBREVIATIONS
ACKNOWLEDGEMENTS
CHAPTER 1 INTRODUCTION AND LITERATURE REVIEW
1.1 GENERAL INTRODUCTION:CYCLODEXTRIN GLYCOSYLTRANSFERASE ENZYMES AND CYCLODEXTRIN PRODUCTION
1.2 CYCLODEXTRIN GLYCOSYLTRANSFERASE ENZYMES
1.2.1 Starch as a Substrate for CGTase Production
1.2.2 Cyclodextrin Glycosyltransferase
1.3 CYCLODEXTRINS(CDS)
1.3.1 Chemical Structure and Properties
1.3.2 Solubility of CDs
1.3.3 Use and Regulatory Status of CDs
1.3.4 CD Production
1.4 PROBLEM STATEMENT AND JUSTIFICATION OF RESEARCH
1.4.1 Problem Statement
1.4.2 Justification of Research
1.5 MAIN OBJECTIVE
1.5.1 Specific Objectives
CHAPTER 2 EFFECT OF MEDIA COMPOSITION ON PRODUCTION AND ACTIVITY OF CGTASE FROM BACILLUS LICHENIFORMIS SK 13.002 STRAIN AND ITS APPLICATION FOR B-CYCLODEXTRIN PRODUCTION
2.1 INTRODUCTION
2.2 MATERIALS AND METHODS
2.2.1 Materials
2.2.2 Bacterial Strain Cultivation Conditions for CGTase Production
2.2.3 Effect of Initial pH on CGTase Production
2.2.4 Effects of Media Composition on CGTase Production
2.2.5 Time Course Production of CGTase
2.2.6 CGTase Activity Assays
2.2.7 CGTase Thermal Stability
2.2.8 Analysis of CGTase CD Production by HPLC
2.2.9 Statistical Analysis
2.3 RESULTS AND DISCUSSION
2.3.1 Bacterial Strain Growth and Effect of Initial pH on CGTase Production
2.3.2 Effect of Carbon Sources on CGTase Production
2.3.3 Effect of Nitrogen Sources on CGTase Production
2.3.4 Effect of Metal Ions on CGTase Production
2.3.5 Time Course Production of CGTase
2.3.6 Effect of pH on Cyclization and Hydrolysis Activities
2.3.7 Effect of Temperature on Cyclization and Hydrolysis Activities
2.3.8 CGTase Thermal Stability
2.3.9 Production of CDs
2.4 CONCLUSIONS
CHAPTER 3 PURIFICATION OF BACILLUS LICHENIFORMIS SK 13.002 CGTASE ISOENZYMES
3.1 INTRODUCTION
3.2 MATERIALS AND METHODS
3.2.1 Materials
3.2.2 CGTase Concentration and Precipitation
3.2.3 Purification of CGTase
3.2.4 Molecular Weight and Protein Quantification
3.3 RESULTS AND DISCUSSION
3.3.1 Concentration and Purification of CGTase
3.3.2 Homogeneity and Molecular Weights of CGTase Isozymes
3.4 CONCLUSIONS
4.GENERAL CONCLUSIONS AND RECOMMENDATIONS
4.1 GENERAL CONCLUSIONS
4.2 RECOMMENDATIONS
5.LIST OF PUBLICATIONS
6.REFERENCES