Cyclodextrin glucosyltransferases for the production of gamma-cyclodextrin

摘要

New cyclodextrin glycosyltransferase (CGTase) muteins that convert starch to cyclodextrins with an increased proportion of gamma -cyclodextrin and have at least 60% of the total CGTase activity of the wild-type enzymes from which they are derived have at least one amino acid deletion between positions 155 and 195, inclusive, in the wild-type sequence, where position 1 is the start of the signal peptide. Also claimed are DNA sequences encoding the muteins.