Isolation of GST-IL13 from E.coli JM109 by Aqueous Two-phase Extraction

摘要

In this paper, we investigated the distribution properties of protein, GST-IL13 and cell fragments in PEG/salt aqueous system, inspected the effects of PEG molecular weight and its concentration, some kinds of salts and their consistency, pH, temperature, and drew up the fittest experimental condition. Upon these works, we further try coupling cell disruption and two-phase aqueous extraction, which resultsi ndicated the yield of objective product increased 5-10