Study of DNA interaction with l-phenyl-2-methyl-aminopropanol containing Palladium compound which reveal radiomodifying and immunostimulatory activity

摘要

The interactions of Palladium acido complex (PhAPH2):[PdCl4] (where PhAPH is l-phenyl-2-methyl-aminopropanol) with DNA in 5 mM NaCl and 0.15 M NaCl solutions were studied by spectrophotometry, circular dichroism, viscometry, flow birefringence, and atomic force microscopy. The significant changes in DNA conformation in complexes with palladium due to intra- and intermolecular cross-linkings were observed by atomic force microscopy. This correlates with data received from other methods giving the information about DNA conformation in a solution. The comparison with Pd-compounds containing other ligands in cation was carried out. The absence of the influence of the cation nature in Palladium complex on the final result of DNA binding with compounds was shown. Preliminary data showed that fPhAPH_2)_2[PdCl_4] exhibits the radioprotection properties.