Affinity-based tagging of protein families with reversible inhinitors:a concept for functional proteomics

摘要

In proteomics.sensitive detection and quantification are conventionally done using different staining methods.However,they are non-selective and often difficult to compare and to reproduce.As a matter of principle it is possible to tag certain proteins covalently with a specific ligand(peptide or organic molecule)bearing a reporter group prior to two-dimensional separation.Subsequently the protein in question can easily be identified,e.g.fluorimetrically,and quantified.Especially tagging of enzymes has been achieved simply by using irreversible enzyme inhibitors(suicide inhibitors).