Antibacterial Activity from Ethanol Extracts and Fractions of Family Asteraceae Leaf Against Bacillus cereus and Vibrio cholera

摘要

The use of plants as traditional medication has been widely used in the community. Asteraceae is a family plant with activity against many diseases. The bioactivity dues to bioactive compounds contained in the plants as flavonoids, alkaloids, terpenoids and saponins. This study aims to determine the antibacterial activity in Asteraceae family plants using the paper disc diffusion method. Antibacterial activity was evaluated using Activity Index (AI) and Proportion Index (PI) calculations. The activity test shows the leaves of Asteraceae family plant is potential as antibacterial agents. According to AI and PI calculation, the most effective antibacterial against B. cereus and V. cholera is the ethyl acetate fraction of Insulin (Tithonia diversifolia) leaves. Bacterial cell morphology was observed using Scanning Electron Microscope (SEM), showing a change in bacterial morphology which are pores formation and cell shrinkage.