Studies of L-Glutamate-Production in Coryneform Bacteria By Using Metabolic-Flux Analysis

摘要

An odhA-disrupted mutant extremely produce glutamate into the medium in the presence of excess amonunts of biotin. This result means the mechanism of glutamate production in coryneform bacteria is irreverent to the permeability of a cell membrane. Furthermore, a oble gene, dtsR, was cloned as a multi-copy suppesser gene of a detergent sensitive mutant derived from a wildtype strai nof coryneform bacteria. DtsR showed significant homologies to osme biotin exzymes. A dtsR-disrupted mutant produce glutamte. The mechanism of glutamate production in coryneform bacteria was discussed.