Multiplex SPRi Screening of Over 500 Antibody Fc-Gamma Receptor Affinities in 48 Hours

摘要

Characterization of Fc-gamma receptor binding to IgGs is an essential step in drug discovery and development, owing to the varied effector function pathways that can be activated by an IgG formatted biologic. Selection of an Fc domain with the preferred activity profile for a given therapeutic strategy often requires characterization of multiple IgG formats to understand binding affinities towards Fc gamma receptors from a mechanism driven perspective. Additionally this characterization must be carried out for each IgG Fc variant against both human receptors as well as those of representative preclinical models. Although surface plasmon resonance (SPR) biosensors are well suited to study these types of binding interactions, they historically have been limited in the ability to screen for affinity rapidly in a combinatorial format.