The use of antibiotics as a growth promoter for chickens may develop resistance to pathogenic bacteria in the digestive tract of chickens. Other negative impacts on food safety came from antibiotic residues. One alternative that can be used as a substitute for antibiotics is probiotics. Probiotics derived from lactic acid bacteria (LAB) is safe beside that it is potential to improve growth and chicken health. This study aimed to obtain LAB isolates from asam durian which is a local food in West Sumatera. This study consisted of several steps i.e., isolation, identification, antagonistic test to patogenic bacteria and isolates survival in the gastrointestinal tracts. Results of this study found that 8 isolates of LAB were morphologically identified with negative catalase and gram-positive bacteria. All LAB isolates had inhibitory activity to Escherichia coli. The diameter of the inhibitory zone was used to select the potential of LAB and further tested for their survival in the digestive tract at pH 2, 4 and 6 and 0.5% bile salts. Isolate P1 and isolate P3 had inhibitory zone diameters of 17.35 mm and 16.08 mm, respectively, which could survive at the pH of 2, 4 and 6 and 0.5% bile salts. It is concluded that isolate P1 and isolate P3 from asam durian have the potential as probiotics for chickens because they inhibit the growth of pathogenic bacteria and can survive in the gastrointestinal conditions.
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