Super-resolution optical microscopy has enabled the observation of ultra-fine structures and features beyond thediffraction limit, among which nonlinear absorption has been a useful tool to investigate physical and biologicalcharacteristics in sub-100 nanometer range. Saturation competition microscopy (SAC), based on nonlinear absorptionprinciple, has been demonstrated to obtain high resolution in either fluorescence or non-fluorescence imaging ofbiological applications. Furthermore, fluorescent nanodiamonds (FNDs) have been widely researched as nitrogenvacancycenters (NVCs) in FNDs are important medium in quantum entanglement. Here, we report on thecharacterization of NVCs using pulse SAC (pSAC) microscopy. Resolution of λ/6 has been reached and theexperimental results shows that it has better signal-to-background ratio (SBR) with lower illumination intensity incontrast to stimulated emission depletion microscopy (STED).
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