A boron-doped diamond electrode decorated with hemoglobin-modified platinum nanoparticles as a biosensor for acrylamide detection

摘要

Acrylamide(AA)is a neurotoxin and potential carcinogen.It has been found in various thermally processed foods,e.g.,potato chips and biscuits.Thus,simple,rapid,and sensitive methods for AA detection are needed to ensure food safety.Herein,the fabrication of a highly stable AA biosensor is presented.A boron-doped diamond(BDD)was modified by Pt and hemoglobin.In the first step,platinum nanoparticles(Pt NPs)were chemically seeded onto the BDD surface using NaBH.as a reducing agent.The electrochemical overgrowth of these Pt NP seeds was conducted at a constant potential of-0.2 V in a 1 mM Pt solution.Then,rapid thermal annealing(RTA)of the BDD/Pt NP composite was conducted at 700 °C under K atmosphere to enhance its stability.After RTA,BDD/Pt NP was eleetrochemically activated between-0.5 and 1.5 V.Then,further overgrowth was performed using a deposition voltage of-0.2 V to renew toe BDD/Pt NP surface.Finally,0.15-mM hemoglobin was used to modify BDD/Pt NP.The characterization of the resulting surface was performed using scanning electron microscopy.The biosensor exhibited an optimal response(limit of detection = 0,012 nM)at pH 4,9 in a 0.2-M acetate buffer solution.