Rapid supercritical fluid chromatography analysis for 18 phthalate esters and bisphenol A in dairy products

摘要

A rapid and environmental method based on supercritical fluid chromatography (SFC) was established for the simultaneous analysis of 18 phthalate esters (PAEs) and bisphenol A (BPA) in dairy products. The stationary phases, organic modifiers, back pressures and column temperatures were investigated for the optimal separation. The BEH chromatographic column (100 mm x 3.0 mm, 1.7 mm) with sub-2-micron particles was performed for the detection of target compounds. 18 PAEs and BPA can be effectively separated within 5.5 min with acetonitrile as solvent modifier at the optimal conditions. The correlation coefficients (R2) of 18 PAEs and BPA were more than 0.997 in the linear range of 0.3-10.0 mg/L, except for BMPP, DNOP and BPA in the range of 0.6-10.0 mg/L. The limits of quantification (LOQs) for PAEs and BPA were 0.15-0.60 mg/kg. The spiked recoveries and relative standard deviation (RSDs) were in the range of 88.2-120.5% and 1.55-13.51% for powdered milk infant formula samples, 89.6-114.2% and 0.69-10.06% for milk drinks samples, respectively. The SFC method can be used as a simple and efficient alternative route for the detection of 18 PAEs and BPA in dairy products.