Detremination of kinetic effect of Metoprolol and Ranitidine on HRP-modified GC electrode biosensor

摘要

Peroxidase is classified as oxidoreductive enzyme which catalyzes the oxidation of the substrate by a hydrogen peroxide solution, the composition of a protein which is the prosthetic group of HEM. In this paper, the effect of active substance from the different groups of drugs on peroxidase activity was studied. Electrochemical tests were performed in classical three-electrode system with techniques of cyclic voltametry and chronoamperometry. Amperometric biosensor for the determination of H2O2 is presented, based on GC electrode on which peroxidase is immobilized in Nation film. Cyclic voltametry technique was used to research the influence of different substrate concentration on the enzyme activity in a potential range between-1,0 V and 0,7 V and scan rate of 50 mV/s. Chronoamperometric technique was used for the determination of kinetic parameters Km and Vmax at a constant potential of 0,9 V.