Expanded Panel of Cytometric Markers for Canine Immune Thrombocytopenia

摘要

Platelet count alone is not predictive of bleeding severity or response to treatment for dogs with ITP. We evaluated dogs with severe thrombocytopenia to identify platelet abnormalities with potential diagnostic or prognostic relevance.Flow cytometric assays detected platelet membrane-bound IgG and IgM (PsAIg), fibrinogen receptor (CD61) expression, and phosphatidylserine (PS) externalization. Microspheres conjugated with defined concentrations of canine immunoglobulin were used as assay controls. To assess sample stability, replicate analyses were performed on EDTA blood samples from healthy dogs (n=7) through 72 hours of storage. Pre-treatment samples were also analyzed within 48 hours of collection from 21 dogs with severe thrombocytopenia (<50,000 platelets/ uL) in 3 categories: primary/autoimmune (PI, n=7); infectious/lymphoproliferative/drug-associated secondary immune (SI, n=7); non-immune (NI, n=7) due to consumption or bone marrow suppression.