Development and Application of a Novel Nucleic Acid Amplification Kit on Detection of MRSA

摘要

Methicillin-resistant Staphylococcus aureus (MRSA) now becomes a global health concern. It costs 3 d-4 d to finish MRSA detection procedure using conventional methods, also it may have false positive or false negative. Thus, developing an accurate and rapid method in MRSA detection and infection control becomes necessary. This study aimed to develop and establish a multiplex PCR assay for rapid and sensitive detection of MRSA. Four genetic loci were selected to be detected in one amplification system, 16S rRNA of Staphylococcus genus, femA of S. aureus, mecA of methicillin-resistance and orfX of SCCmec. The PCR reaction was finished within 3 h. The specificity of multiplex PCR was brought out with the evaluation using four different kinds of reference strains including MSCNS, MRCNS, MSSA and MRSA. The diagnostic evaluation was brought out with the appliance of 33 clinical MRSA strains. The results showed that the sensitivity and diagnostic rate of multiplex PCR was 100% and without false negative or false positive. To sum up, this rapid detection method has the potential in the diagnosing and infection control of MRSA.