Cloning and Expression Analysis of CER1 in Blackberry (Rubus Spp.)

摘要

To understand the molecular mechanisms of cuticle wax formation in blackberry, the waxy synthetic related gene CER1 was cloned from blackberry. The open reading frame of this gene was 1878 bp, encoding 625 amino acid residues with a molecular weight of 72.3 kD and a theoretical isoelectric point of 8.59. Its amino acid sequence has the highest identity with strawberry. The results of real-time quantitative PCR showed that the gene expression increased with the maturation of blackberry. With the increase of the ratio of red or blue light, the expression of CER1 gene firstly decreased and then increased. Under the condition of red and blue light at a ratio of 2:8, the expression of CER1 was the lowest. The reason may be related to the different mechanisms of photoreceptor response to red and blue light.