Cloning vectors and their preparation and use for mRNA expression pattern analysis

摘要

Disclosed herein are cloning vectors which include:;(a) a cloning site which permits the cloning of a nucleic acid in defined orientation;;(b) at least one cleavage site adjacent to the cloning site, the cleavage site being rarely-occurring in nucleic acids; and;(c) a long region which is located on the side of the cloning site opposite to the cleavage site (b), wherein the long region and the region between the cloning site and the cleavage site (b) contain neither the cloning site nor at least two frequently-occurring cleavage sites.