CLONING VECTOR, ITS PREPARATION AND USE FOR MRNA EXPRESSION PATTERN ANALYSIS

摘要

The invention relates to a cloning vector containing(a) a cloning site which permits the cloning of a nucleic acid in definedorientation,(b) at least one cleavage site adjacent to the cloning site (a) and onlyrarely present innucleic acids,(c) a long region which is located on the side of the cloning site (a)opposite to thecleavage site (b), where the long region and the region between the cloningsite(a) and the cleavage site (b) contains neither the cloning site nor at leasttwocleavage sites frequently present in nucleic acids.