Cloning vector, its preparation and use for the analysis of mrna expression pattern

摘要

The invention relates to a cloning vector containing (a) a cloning site which permits the cloning of a nucleic acid in defined orientation, (b) at least one cleavage site adjacent to the cloning site (a) and only rarely present in nucleic acids, (c) a long region which is located on the side of the cloning site (a) opposite to the cleavage site (b), where the long region and the region between the cloning site (a) and the cleavage site (b) contains neither the cloning site nor at least two cleavage sites frequently present in nucleic acids.