Research on Effects of Herb of Glabrous Sarcandra on Human Prostate Carcinoma DU-145 Cells PI3K/m TOR Signal Transduction Pathway

摘要

In order to investigate the effect of herb of glabrous sarcandra solution on human prostate cancer DU-145 cells PI3K/m TOR signal transduction pathway, the paper uses method of cell culture, with Sarcandrae solution with different concentrations of 48h, the impact on DU-145 cells were detected by MTT solution herb of glabrous sarcandra method of DU-145 cell proliferation by RT - PCR detected after treatment cells PI3K, mTOR and P70S6K gene expression. Herb of glabrous sarcandra action of wind solution after 48h, DU-145 cell proliferation was inhibited and showed dose-effect relationship; DU-145 cells expressing the P13K, mTOR and P7OS6K showed different degrees of inhibition, it does not show a dose relationship. This indicates herb of glabrous sarcandra solution can inhibit human prostate cancer DU-145 cell proliferation, cell proliferation inhibition mechanism related to the inhibition of cell P13K/mTOR signaling pathway effects.