The voltammetric study of β-glucosidase hydrolytic product With modified electrode

摘要

The novel modified electrode of voltammetric technique was applied to detect p-nitrophenol quantity released from the synthetic substrate p-nitrophenyl-P-D-glucopyranoside (pNPG) for p-glucosidase activity measurement. The results from the developed bismuth film modified screen printed carbon electrode (Bi/SPCE) for p-nitrophenol detecting, can be used for enzyme activity assay during purification steps. The further work, the condition of convenient voltammetric method will be optimized the condition suitable for applying to the P-glucosidase enzyme purification from Terminalia ivorensis C. seeds.