Salmonella Detection on Microfluidic CD Using Loop Mediated Isothermal Amplification

摘要

Salmonella is a major food borne pathogen threat and an economic burden for the food business, individuals and society. Therefore, low-cost, portable, and easy-to-use devices for Salmonella detection are required to prevent foodborne outbreaks and ensure food safety. This paper describes Salmonella detection on centrifugal microfluidic compact disc (CD) using Loop Mediated Isothermal Amplification (LAMP) technique. LAMP amplification and Salmonella detection are simultaneously integrated on this developed centrifugal microfluidic device. A cheap hot air gun was used to produce a temperature for the DNA amplification resulting in system miniaturization and low cost. The whole process from reagent preparation, Salmonella DNA amplification and detection was automatically performed on the microfluidic CD and SYBR Green I was used for detection process. Our system offers a rapid and automated diagnostic platform. Therefore, reducing the usage of manual and expensive operated equipment, that subsequently could reduce manpower, shortened time-to-result, and lower the cost.