Phytochemistry and Antioxidant Activity of Molineria latifolia Herb. Extracts

摘要

Molineria latifolia is a perennial-monocotyle-donous herb and it is well-known in the treatment of melasma, freckle and black spot in Thai traditional medicine. The purpose of this study was to determine the phytochemicals and antioxidant activity in the aerial parts and the underground parts of M. latifolia by water, ethanolic and ethyl acetate extracts. The phytochemicals were analyzed by HPTLC. The antioxidant activity was measured by using the DPPH radical scavenging activity assay, Ferric reducing antioxidant power assay and Total phenolic content was measured by Folin-Ciocalteu. The result showed the highest antioxidant activity of the underground parts (RW) were extracted by water at EC_(50)=245.03±6.59 μg/mL (DPPH) and 640.03±0.36 Fe~(2+)/mg (FRAP). Consequently, the highest phenolics content was found in the water extract of the aerial parts (LW) (191.70±1.64 μg FA/mg). M. latifolia extracts contain a bioactive compound of Tannin, Flavonoids and Steroid. Tannin and Flavonoid were found in both of parts. In the aerial parts were found steroid extracting by ethyl acetate and ethanolic, as the underground parts were seen steroid extracting by water. It was found that M. latifolia has high bioactive compounds and moderate antioxidant activity. These results for the cosmetic product development in the future.