Antioxidant activity of collagen hydrolysate obtained from tilapia scales in vitro and in vivo assays

摘要

Collagen hydrolysate of S and C were prepared by enzymatic hydrolysis decalcified tilapia scale and pepsin-solubilised collagen extracted from decalcified tilapia scale. The antioxidative activity of C and S were evaluated by their effects on scavenging ability of superoxide anion O~(2-) radicals, 1, 1-diphenyl-2-picrylhydrazyl (DPPH) radicals and reductive ability in vivo. Besides, the activity of glutathione peroxidase (GSH-Px), total antioxidant capacity (T-AOC) and content of malondialdehyde (MDA) in vitro of the laboratory mouse were detected. The results indicated that collagen hydrolysate of C and S show prominent inhibitory effect on scavenging O~(2-) with the fifty-percent inhibitory concentration (IC_(50)) of 3.7 and 6.5 mg/mL, respectively. The IC_(50) of collagen hydrolysate of C and S inhibit the DPPH were 8.4 and 7.2 mg/mL, respectively. Two collagen hydrolysates demonstrated highly reducing activity. In vivo experimentation suggested that C and S can significantly improve the GSH-Px activity, T-AOC and reduce the contents of MDA. As a consequence of results, collagen hydrolysates of C and S extracted from decalcified tilapia scale have a good antioxidation.