An Ultra Fast Cooling Method for Cell Vitrification Cryopreservation Utilizing Thin Film Evaporation

摘要

Ultra-fast cooling is the key to successful cell vitrification cryopreservation of lower concentration cryoprotective solution. This research develops a cell cryopreservation methodology which utilizes thin film evaporation and achieves vitrification of relatively low concentration cryoprotectant with an ultra-fast cooling rate. Experimental results show that the average cooling rate of dimenthylsulphoxide cryoprotective solution reaches 150,000°C/min in a temperature range from 10°C to -180°C. The ultra-fast cooling rate can remarkably improve the vitrification tendencies of the cryoprotective solution. This methodology opens the possibility for more successful cell vitrification cryopreservation.