Extraction of Effective Compounds in Pine Pollen and in Vitro Assay of Their Antitumor Effect

摘要

Supercritical CO_2 was used to extract effective compounds from pine pollen, MTT colorimetric method was applied to determine anti-cancer effect of effective compounds in pine pollen. Results indicated that extracts obtained at pressure ranges 0~30 MPa and 10~15 MPa had significant inhibition effect on the lung cancer cells 95D of pig (95D) and their IC50 value was 253.22 ìg/mL and 252.19 ug/mL, respectively. Extracts attained at pressure ranging 0~10 MPa, 10~15 MPa and 0~30 MPa had significant inhibition effect on the stomach cancer cells SGC-7901 of human (SGC-7901), and their IC_(50) value was 248.35 ìg/mL, 459.56 ìg/mL and 301.20 ìg/mL, individualy. Supercritical CO_2 extract of pine pollen had inhibition effects on the lung cancer cells 95D of pig and the stomach cancer cells SGC-7901 of human, and the corresponding active compounds were different.