Detection of 9a-OH-AD prepared by biotransformation

摘要

Detection of 9a-OH-AD prepared by biotransformation by RP- HPLC directly was studied. The detection is performed on a Kromasil 100-5C 18 (4.6×250mm) column, using methanolrwater (7:3,v/v) as mobile phase,0.8mL min~(-1) flow rate and external standard method,detected at 242nm .There is a good line correlation between peak area and content in range of 0.01-0.20 g/L, the correlation coefficient is 0.9942, the average recovery is 99.09% with a relative stand deviation of 0.89% (n=5). The method is simple, stable, accurate and reliable for quality control of 9a-OH-AD.