Preparation of Guiqi Polysaccharide and antioxidant activity in vitro

摘要

Polysaccharide extracts (ASP, AMP and GQP) were obtained by water extraction and ethyl alcohol deposition method from Angelica sinensis, Astragalus membranaceus and the mixture of Angelica sinensis and Astragalus membranaceus. And the total carbohydrate content of AMP, ASP and GQP was respectively determined to be 63.1%, 48.7% and 76.7% by phenol-sulfuric acid colorimetric method using glucose as the standard. The in vitro antioxidant activities of 3 polysaccharides were evaluated by free radical, superoxide radicals and DPPH free radical scavenging assay, respectively. Among 3 polysaccharides, GQP showed the highest antioxidant activity in vitro at 100-300μg/mL. These results indicated that the GQP might be useful for developing natural safe antiaging drugs or health food.