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Chemical analysis and antioxidant activities in vitro of polysaccharide extracted from corn silk

机译:玉米丝提取的多糖体外化学分析及抗氧化活性

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Corn silk polysaccharide (CSP) was investigated for the treatment of different kinds of diseases. In order to characterize the chemical properties and antioxidant activities of CSP, the CSP was isolated from corn silk and purified by DE-52 cellulose column chromatography. Four components were separated, and the highest one named CSP-A. The CSP-A was characterized by FTIR and the monosaccharide components were analyzed by HPLC. The FTIR spectra indicated that CSP-A was characteristic of β-glucosidic bond and α-glycosidic bond. The CSP-A mainly comprised of glucose, galactose, arabinose, mannose, rhamnose. The antioxidant activities of CSP were determined by hydroxyl radical (·OH) and DPPH radicals scavenging assays. When the concentration of CSP was 10mg/mL, the scavenging capacity of ·OH and DPPH could reach to 40% and 48%, respectively.
机译:研究了玉米丝多糖(CSP)以治疗不同种类的疾病。为了表征CSP的化学性质和抗氧化活性,CSP与玉米丝分离,并通过DE-52纤维素柱色谱纯化。分开了四个组分,最高的组件名为CSP-A。 CSP-A的特征在于FTIR,通过HPLC分析单糖组分。 FTIR光谱表明CSP-A是β-葡糖苷键和α-糖苷键的特征。 CSP-A主要由葡萄糖,半乳糖,阿拉伯糖,甘露糖,鼠李组成。 CSP的抗氧化活性由羟基自由基(·OH)和DPPH基团清除测定法测定。当CSP的浓度为10mg / ml时,·OH和DPPH的清除能力分别达到40%和48%。

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