LC/Ultraviolet/Fluorescence and LC/MS/MSAnalyses of Multiple Mycotoxins in SpikedBeer Using Immunoaffinity Column Clean-Up

摘要

Ten different mycotoxins namely deoxynivalenol (DON), aflatoxins B_1, B_2, G_1, G_2, fumonisins B_1, B_2, B_3, zearalenone (ZON), and ochratoxin A (OTA) present in fortified beer matrices were extracted using mixed-bed ("Myco5inl") Immunoaffinity columns and were detected using both the classical LC/Ultraviolet/Fluorescence with post-column derivatization and modern LC/MS/MS techniques. HPLC separations were performed using a reverse phase C_(18) column. Mass Spectroscopy measurements were done in the positive ESI mode. Multiple reaction monitoring (MRM) analyses were performed for each mycotoxin. The LC/u.v./Fluorescence method detection limit (MDL) ranged from 0.02 to 6 ppb and the LC/MS/MS MDL ranged from 0.5 to 2.5 ppb, respectively. The recoveries of multi-mycotoxin fortified triplicate beer samples using the "Myco5inl" column ranged from 73% to 102%. Both analytical methods achieved good separations and detections.