Expression of MTS1 Gene and Its Protein Product in Laryngeal Cancer

摘要

The MTS1/p16INK4a gene has been identified as a cyclin-dependent kinase inhibitor and may negatively regulate the cell cycle acting as a tumour suppressor. Our purpose was to investigate 9p21 LOH and expression of pl6 protein and their possible prognostic value in laryngeal cancer. 41 patients with laryngeal cancer was analysed. PCR-based techniques were used for investigating 9p21 LOH. Paraffin-embedded tissue sections were immunohistochemically stained with a monoclonal antibody raised against pl6 protein. 9p21 LOH was detected in 10/41 (24,4%) informative tumours. Expression of pl6 protein was observed in 11/41 cases (26,8%) and was significantly correlated with T status (p=0,045), N status (p=0,035) and nodal recurrences (p=0,015). By univariate analysis 9p21 LOH proved to be significantly related to overall survival whereas expression of pl6 protein was related with quicker relapse. Analysis of 9p21 LOH enables the assessment of biology of laryngeal cancer, it can be a prognostic factor of overall survival and pl6 protein has a prognostic value in assessment of disease free survival.