Physico-chemical parameters and in-situ bacterial flora by novel PCR (Polymeraze Chain Reaction) method for improvement of composting

摘要

Composting has been carried out for centuries with very limited analytical information having been reported on the relationship between physico -chemical phenomena and microorganisms. According to recent research works, it is said that previous information based on culture methods may not represent the actual flora working important roles in composting process. In this paper, the PCR (Polymeraze Chain Reaction) Method, which is now becoming popular method for genetic analysis in DNA fractions was applied to DNA extracted from microorganisms working in biodegradation of organic wastes during of composting. And a novel PCR method, so called SSC (Single Strain Counting)-PCR, was developed for analysis in the microbial flora of composting process. Theexperimental results showed that large diversity in microbial flora from time to time than ever expected was observed and that micro organisms could adapt to different environment conditions provided by composting process. By the comparison between physico - chemical factors and information from this method, we can obtain more detail information relating to microorganisms working major roles for more efficient and stable composting of biological wastes.