One embodiment of the present invention relates to a PCR apparatus comprising a repeated sliding means and a PCR method using same. According to the present invention, the throughput of samples can be increased by simultaneously, rapidly, and accurately performing a PCR on the large number of samples through repeated thermal contact between a PCR heating block having two or more heaters disposed therein and a PCR chip having two or more reaction chambers disposed therein. In addition, the present invention is capable of: significantly improving PCR yield by preventing radial heat distribution generated by the individual heaters and the consequent nonuniform thermal overlap between the adjacent heaters; significantly contributing to the miniaturization and integration of the apparatus by requiring no separate temperature control means; furthermore, simultaneously and rapidly amplifying multiple nucleic acid samples by using the PCR heating block having heater units repeatedly disposed therein and a plate-shaped PCR chip; and checking the process of nucleic acid amplification in real time by measuring sequential optical signals or electrochemical signals.
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