Proof of Porster Energy Transfer between FAD and PMN in Cytochrome P450 Reductase by Time-Resolved Red-Edge Spectroscopy.

摘要

By use of steady state and time-resolved fluorescence techniques Forster energy transfer between the cofactors, FAD and FMN. in cytochrome-P450 reductase is observed. Red-edge spectroscopy allowed us to assign the actual rate constant of transfer as apparent in the anisotropy decay of the holoenzyme. The same approach was used in biflavinyl compounds entrapped in polymethylmetacrylate. As anticipated the fluorescence depolarisation of this system showed a great similarity with the enzyme system. From the results the interflavin distance in the enzyme was estimated between 1.6 and 2.4 nm.