METHOD OF MEASURING NADPH-CYTOCHROME P450 REDUCTASE ACTIVITY IN A SAMPLE USING 5-CYANO-2,3-DITOLYL TETRAZOLIUM CHLORIDE AS SUBSTRATE, METHOD OF SCREENING A MATERIAL MODULATING NADPH-CYTOCHROME P450 REDUCTASE ACTIVITY USING THE METHOD, AND KIT FOR MEASURING NADPH-CYTOCHROME P450 REDUCTASE ACTIVITY

摘要

Provided are a method of measuring CPR activity in a sample, the method including: reacting a sample including an enzyme having an NADPH-cytochrome P450 reductase (CPR) activity with 5-cyano-2,3-ditolyl tetrazolium chloride (CTC) in the presence of NADPH, thereby converting CTC into CTC-formazan; and measuring the concentration of the CTC-formazan by using a spectrophotometric method or a spectrofluorometric method, a method of screening a material for modulating CPR activity by using the method of measuring CPR activity, and a kit for measuring CPR activity in a sample.