USING CYCLIC VOLTAMMETRY TO DISCERN PROTEIN MECHANISM AND CORRELATE STRUCTURE WITH FUNCTION.

摘要

The development of electrochemical biosensors has hinged on the ability to obtain and sustain an active layer of the desired protein at a suitable electrode.This layer must be stable under a wide range of conditions and demonstrate a high degree of specificity for the substrate.Over the past few years,we have explored a range of surface modification techniques that result in stable protein films,enabling direct electrochemical experiments that have led to novel mechanistic information.