A High Throughput, Multiplexed Assay to Facilitate Detection of Shiga Toxin Producing E. coli (STEC) in the Beef Chain

摘要

Ongoing independent test in collaborating laboratories - Assay transfered to Bosilevac lab at USDA; - Assist in other STEC studies. Implement reduced sample processing - Ligation and PCR amplification. Test limits of detection for each probe - Preliminary data showing 10~4 - 10~5 CFU in spiked fecal samples. Expand assay probes - There are 17 subtypes of eae that show agreement with serogroup; - Add in genes EHEC-hlyA, ehx.