A HIGH-THROUGHPUT DRUG-SCREENING SYSTEM TARGETING ABC-TRANSPORTERS: AN APPLICATION OF A MICROFLUIDIC GRADIENT GENERATOR

摘要

We describe a high-throughput methodology to analyze the function of ATP binding cassette transporter (ABC-transporter) by using a microfluidic device that generates a concentration gradient of substrates. Taking advantage of the basic procedure that we established for functional analysis of ABC-transporters, we here show an advanced system utilizing multi-channel microfluidic device. We applied a microfluidic gradient generator, which makes various concentrations of substrates in a single device. After incubation of ABC-transporters at 5 different substrate concentrations in a gradient generator, we succeeded in detecting the difference of substrate transport revealed by the different fluorescence intensity from ABC-transporter vesicles.