RAPID, INDEPENDENTLY CONTROLLED POLYMERASE CHAIN REACTION VIA MULTIPLEXED LASER RADIATION

摘要

We report the design and testing of an instrument implementing thermal multiplexing for rapid microfluidic amplification of multiple DNA targets with different optimal annealing temperatures. Through spatial modulation of an infrared laser, distinct temperature profiles can be generated simultaneously in an array of microfluidic reaction chambers in a polymer device. For our dual-chamber system, temperature differences up to 15°C have been achieved. Such multiplexed temperature control has not been reported to date.