PROCESS ENABLING SIMULTANEOUS DETECTION OF TWO TRANSGENES NAMELY 5-ENOLPYRUVYLSHIKIMATE-3-PHOSPHATE SYNTHASE (EPSPS OR CP4EPSPS) GENE AND CAULIFLOWER MOSAIC VIRUS (CAMV) 35S PROMOTER USING A MULTIPLEX POLYMERASE CHAIN REACTION UTILIZING A COMBINATION OF NOVAL PRIMERS AND POLYMERASE CHAIN REACTION (PCR) PROGRAMME IN TRANGENIC MAIZE

摘要

The present invention comprises a process enabling simultaneous detection of two transgenes using a Multiplex Polymerase Chain Reaction (MPCR), particularly the detection of 5-enolpyruvylshikimate-3-phosphate synthase (EPSPS or CP4EPSPS) gene (which imparts resistance against herbicide glyphosate) and cauliflower mosaic virus (CaMV) 35S promoter gene in transgenic maize for herbicide tolerance. The invention can be successfully employed in rapid and simultaneous detection of these two transgenes in transgenic maize with high reproducibility and sensitivity.