MICROFLUIDIC ASSAY TO STUDY TRANSENDOTHELIAL MIGRATION OF HUMAN LEUKOCYTE

摘要

Most of the study on transendothelial migration has been conducted with 2D in vitro assays, which are not appropriate for high-resolution imaging of cell-cell interaction, quantification of the cell migration ability in time-dependency, and investigation of 3D cell migration. For these reasons, the complex transendothelial migration cascades have hardly ever discovered. To overcome these drawbacks of several established in vitro essays, here, we introduce the hydrogel scaffold embedded microfluidic platform. In this study, we have demonstrated the transendothelial migration of human leukocytes interacting with hMVECs monolayer with the precisely regulated chemotactic gradient of inflammatory mediators.