Construction of elastin-like peptide expression vector using BbsI restriction enzyme for neural regeneration

摘要

In the present experiments, we tried to construct an expression vector encoding poly(VPGIG) with AG73 (RKRLQVQLSIRT) which promotes neurite outgrowth. DNA encoding poly(VPGIG) was elongated via non-palindrome BanI sticky-end and was induced into expression vector using BbsI restriction enzyme. AG73-(VPGIG)_(30) was obtained by E. coli expression and it showed temperature transition and neurite outgrowth of PC12 cells.