Metal-binding Ability of Peptides Originating from Prion Protein by a Column Switch HPLC

摘要

In the cause of the prion disease, Cu~(2+) is thought to be concerned in denaturation of prion protein from normal type to the anomaly. In this study, we test the binding abilities of nine synthetic peptides to various metal ions by a column switch HPLC system. One octarepeat region (OP-l.PHGGGWGQ) binds to only Ni~(2+) and Zn~(2+). Two (OP-2), three (OP-3) and four octarepeat regains (OP-4) bind to Cu~(2+), Ni~(2+), Zn~(2+) and Co~(2+). In addition, the other peptides tested here also bind mainly Cu~(2+). This indicates the prion protein possesses many Cu~(2+) binding site.